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KMID : 0379119840120010035
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Korean Journal of Mycology
1984 Volume.12 No. 1 p.35 ~ p.43
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Studies on Antitumor Components of Cultured Basidiomycetes
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Kim Sung-Won
Kim Byong-Kak
Choi Eung-Chil
Shim Mi-Ja
Kim Yoo-Jin
Lee Chong-Ock
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Abstract
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To produce and characterize antineoplastic constituents in the submerged cultured-mycelia of Laccaria laccata, the mycelia were extracted with distilled water. Purification of the extract was carried out by acetone precipitation, by ion exchange chromatography using DEAE-Sephadex A-50, CM-Sephadex C-25 resins, and by gel filtration chromatography on Sephadex G-200. Each fraction obtained during the purification was examined for antineoplastic activity against sarcoma 180 in ICR mice. As the purification proceeded, the antineoplastic activity was markedly increased. The highly purified Fraction E showed 75% tumor inhibition ratio at a dose of 10㎎/㎏/day and contained 81% polysaccharide and 4% protein. The antitumor component of Fraction E stimulated an accumulation of peritoneal exudate cells including peritoneal macrophages, and is named laccaran.
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